Review



expression plasmid pmscv puro ires gfp 64  (Addgene inc)


Bioz Verified Symbol Addgene inc is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93

    Structured Review

    Addgene inc expression plasmid pmscv puro ires gfp 64
    Expression Plasmid Pmscv Puro Ires Gfp 64, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 80 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/gfp+empty+vector/pMSCV+PIG+(Puro+IRES+GFP+empty+vector)+(Plasmid+%2321654)/10__1016_slash_j__isci__2026__115182-641-41-45
    Average 93 stars, based on 80 article reviews
    expression plasmid pmscv puro ires gfp 64 - by Bioz Stars, 2026-11
    93/100 stars

    Images

    Related Articles

    Transfection:

    Article Title: Dsg2 via Src-mediated transactivation shapes EGFR signaling towards cell adhesion
    Article Snippet: .. 50,000 cells were seeded and transfected right away with 1 μg endotoxin-free Dsg2–GFP plasmid DNA or the GFP empty vector [ 98 ] (pDEST-eGFP-N1, Addgene plasmid # 31796, gift from Robin Shaw, Cedars-Sinai Heart Institute, Los Angeles, USA) using TurboFectTM (Life Technologies) according to the manufacturer’s protocol and incubated for 48 h. Medium was changed 8 h after transfection. ..

    Article Title: The role of APC-mediated actin assembly in microtubule capture and focal adhesion turnover.
    Article Snippet: Protein levels were detected and quantified by band densitometry using a LI-COR Odyssey Infrared Imaging System (LI-COR Biotechnology). .. For immunoprecipitations (Fig. 6 I and Fig. S5 E), cells were transfected as described above with plasmids expressing fulllength APC (WT or m4), GFP-LC3 (11546; Addgene), GFPLAMP1 (34831; Addgene), and/or GFP empty vector (54522; Addgene). .. 12 h after transfection, cells were pelleted and resuspended in lysis buffer (150 mM NaCl, 0.1% NP-40, 50 mM Tris-Cl, pH 7.5, 1 mM EDTA, 10% glycerol, 1 mM sodium orthovanadate, 60 mM β-glycerophosphate, 50 mM sodium fluoride, 1 mM PMSF, and 1× Roche complete protease inhibitor mixture), and then incubated for 30 min at 4°C with vortexing every 2 min.

    Article Title: Survival motor neuron protein deficiency impairs myotube formation by altering myogenic gene expression and focal adhesion dynamics
    Article Snippet: .. Two micrograms of GFP-SMN or GFP empty vector or GFP-talin (Addgene, Cambridge, MA, USA) was used for each transfection. .. Muscle cells were lysed in 1% NP-40, 50 mM Tris–HCl (pH 8), 150 mM NaCl and protease inhibitor cocktail (Roche, Indianapolis, IN, USA) on ice for 10 min.

    Article Title: Dsg2 via Src-mediated transactivation shapes EGFR signaling towards cell adhesion.
    Article Snippet: Rapidly renewing epithelial tissues such as the intestinal epithelium require precise tuning of intercellular adhesion and proliferation to preserve barrier integrity.. Here, we provide evidence that desmoglein 2 (Dsg2), an adhesion molecule of desmosomes, controls cell adhesion and proliferation via epidermal growth factor receptor (EGFR) signaling.. Dsg2 is required for EGFR localization at intercellular junctions as well as for Src-mediated EGFR activation.

    Plasmid Preparation:

    Article Title: Dsg2 via Src-mediated transactivation shapes EGFR signaling towards cell adhesion
    Article Snippet: .. 50,000 cells were seeded and transfected right away with 1 μg endotoxin-free Dsg2–GFP plasmid DNA or the GFP empty vector [ 98 ] (pDEST-eGFP-N1, Addgene plasmid # 31796, gift from Robin Shaw, Cedars-Sinai Heart Institute, Los Angeles, USA) using TurboFectTM (Life Technologies) according to the manufacturer’s protocol and incubated for 48 h. Medium was changed 8 h after transfection. ..

    Article Title: The role of APC-mediated actin assembly in microtubule capture and focal adhesion turnover.
    Article Snippet: Protein levels were detected and quantified by band densitometry using a LI-COR Odyssey Infrared Imaging System (LI-COR Biotechnology). .. For immunoprecipitations (Fig. 6 I and Fig. S5 E), cells were transfected as described above with plasmids expressing fulllength APC (WT or m4), GFP-LC3 (11546; Addgene), GFPLAMP1 (34831; Addgene), and/or GFP empty vector (54522; Addgene). .. 12 h after transfection, cells were pelleted and resuspended in lysis buffer (150 mM NaCl, 0.1% NP-40, 50 mM Tris-Cl, pH 7.5, 1 mM EDTA, 10% glycerol, 1 mM sodium orthovanadate, 60 mM β-glycerophosphate, 50 mM sodium fluoride, 1 mM PMSF, and 1× Roche complete protease inhibitor mixture), and then incubated for 30 min at 4°C with vortexing every 2 min.

    Article Title: Dsg2 via Src-mediated transactivation shapes EGFR signaling towards cell adhesion.
    Article Snippet: Rapidly renewing epithelial tissues such as the intestinal epithelium require precise tuning of intercellular adhesion and proliferation to preserve barrier integrity.. Here, we provide evidence that desmoglein 2 (Dsg2), an adhesion molecule of desmosomes, controls cell adhesion and proliferation via epidermal growth factor receptor (EGFR) signaling.. Dsg2 is required for EGFR localization at intercellular junctions as well as for Src-mediated EGFR activation.

    Incubation:

    Article Title: Dsg2 via Src-mediated transactivation shapes EGFR signaling towards cell adhesion
    Article Snippet: .. 50,000 cells were seeded and transfected right away with 1 μg endotoxin-free Dsg2–GFP plasmid DNA or the GFP empty vector [ 98 ] (pDEST-eGFP-N1, Addgene plasmid # 31796, gift from Robin Shaw, Cedars-Sinai Heart Institute, Los Angeles, USA) using TurboFectTM (Life Technologies) according to the manufacturer’s protocol and incubated for 48 h. Medium was changed 8 h after transfection. ..

    Article Title: Dsg2 via Src-mediated transactivation shapes EGFR signaling towards cell adhesion.
    Article Snippet: Rapidly renewing epithelial tissues such as the intestinal epithelium require precise tuning of intercellular adhesion and proliferation to preserve barrier integrity.. Here, we provide evidence that desmoglein 2 (Dsg2), an adhesion molecule of desmosomes, controls cell adhesion and proliferation via epidermal growth factor receptor (EGFR) signaling.. Dsg2 is required for EGFR localization at intercellular junctions as well as for Src-mediated EGFR activation.

    Expressing:

    Article Title: The role of APC-mediated actin assembly in microtubule capture and focal adhesion turnover.
    Article Snippet: Protein levels were detected and quantified by band densitometry using a LI-COR Odyssey Infrared Imaging System (LI-COR Biotechnology). .. For immunoprecipitations (Fig. 6 I and Fig. S5 E), cells were transfected as described above with plasmids expressing fulllength APC (WT or m4), GFP-LC3 (11546; Addgene), GFPLAMP1 (34831; Addgene), and/or GFP empty vector (54522; Addgene). .. 12 h after transfection, cells were pelleted and resuspended in lysis buffer (150 mM NaCl, 0.1% NP-40, 50 mM Tris-Cl, pH 7.5, 1 mM EDTA, 10% glycerol, 1 mM sodium orthovanadate, 60 mM β-glycerophosphate, 50 mM sodium fluoride, 1 mM PMSF, and 1× Roche complete protease inhibitor mixture), and then incubated for 30 min at 4°C with vortexing every 2 min.

    other:

    Article Title: The role of APC-mediated actin assembly in microtubule capture and focal adhesion turnover
    Article Snippet: Protein levels were detected and quantified by band densitometry using a LI-COR Odyssey Infrared Imaging System (LI-COR Biotechnology).



    Similar Products

    93
    Addgene inc expression plasmid pmscv puro ires gfp 64
    Expression Plasmid Pmscv Puro Ires Gfp 64, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/gfp+empty+vector/pMSCV+PIG+(Puro+IRES+GFP+empty+vector)+(Plasmid+%2321654)/10__1016_slash_j__isci__2026__115182-641-41-45
    Average 93 stars, based on 1 article reviews
    expression plasmid pmscv puro ires gfp 64 - by Bioz Stars, 2026-11
    93/100 stars
      Buy from Supplier

    93
    Addgene inc addgene 209057 pdonr223 gfp
    Addgene 209057 Pdonr223 Gfp, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/gfp+empty+vector/Luc%2ECre+empty+vector+(Plasmid+%2320905)/bio_rxiv__2025__10__17__683176-162-42-42
    Average 93 stars, based on 1 article reviews
    addgene 209057 pdonr223 gfp - by Bioz Stars, 2026-11
    93/100 stars
      Buy from Supplier

    93
    Addgene inc pmscv pig
    Pmscv Pig, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/gfp+empty+vector/pMSCV+PIG+(Puro+IRES+GFP+empty+vector)+(Plasmid+%2321654)/bio_rxiv__2025__09__04__674312-254-19-23
    Average 93 stars, based on 1 article reviews
    pmscv pig - by Bioz Stars, 2026-11
    93/100 stars
      Buy from Supplier

    96
    OriGene pcmv6 ac gfp empty vector
    Pcmv6 Ac Gfp Empty Vector, supplied by OriGene, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/gfp+empty+vector/pCMV6-AC-GFP+Mammalian+Expression+Vector/10__1016_slash_j__jes__2025__09__017-88-1-9
    Average 96 stars, based on 1 article reviews
    pcmv6 ac gfp empty vector - by Bioz Stars, 2026-11
    96/100 stars
      Buy from Supplier

    93
    Addgene inc expression plasmid pmscv puro ires gfp
    Expression Plasmid Pmscv Puro Ires Gfp, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/gfp+empty+vector/pMSCV+PIG+(Puro+IRES+GFP+empty+vector)+(Plasmid+%2321654)/bio_rxiv__2025__08__18__670812-132-39-42
    Average 93 stars, based on 1 article reviews
    expression plasmid pmscv puro ires gfp - by Bioz Stars, 2026-11
    93/100 stars
      Buy from Supplier

    93
    Addgene inc mammalian expression plasmid pmscv puro ires gfp
    Mammalian Expression Plasmid Pmscv Puro Ires Gfp, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/gfp+empty+vector/pMSCV+PIG+(Puro+IRES+GFP+empty+vector)+(Plasmid+%2321654)/pmc12337194__BLOODA_ADV-2024-015627-mmc1-4-42-46
    Average 93 stars, based on 1 article reviews
    mammalian expression plasmid pmscv puro ires gfp - by Bioz Stars, 2026-11
    93/100 stars
      Buy from Supplier

    93
    Addgene inc china n a pmscv gfp addgene
    China N A Pmscv Gfp Addgene, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/gfp+empty+vector/pMSCV+PIG+(Puro+IRES+GFP+empty+vector)+(Plasmid+%2321654)/pmc12234735__41467_2025_61552_MOESM1_ESM-4-38-41
    Average 93 stars, based on 1 article reviews
    china n a pmscv gfp addgene - by Bioz Stars, 2026-11
    93/100 stars
      Buy from Supplier

    93
    Addgene inc pmscv pig cloning vector
    Pmscv Pig Cloning Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/gfp+empty+vector/pMSCV+PIG+(Puro+IRES+GFP+empty+vector)+(Plasmid+%2321654)/pmc12166406-480-16-19
    Average 93 stars, based on 1 article reviews
    pmscv pig cloning vector - by Bioz Stars, 2026-11
    93/100 stars
      Buy from Supplier

    93
    Addgene inc pmscv pig vector
    Pmscv Pig Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/gfp+empty+vector/pMSCV+PIG+(Puro+IRES+GFP+empty+vector)+(Plasmid+%2321654)/bio_rxiv__2025__06__02__657432-214-14-16
    Average 93 stars, based on 1 article reviews
    pmscv pig vector - by Bioz Stars, 2026-11
    93/100 stars
      Buy from Supplier

    Image Search Results